|Application ||WB, IHC|
|Other Names||Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase, AMACR|
|Target/Specificity||Full length human recombinant AMACR protein|
|Application Note||Western Blotting : 1:100-1:200 for 2 hours at RT|
Immunohistology (formalin-fixed) : 1:50-1:100 for 30-60 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml of Culture Supernatant with azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||AMACR / p504S (Prostate Cancer Marker) Antibody - Culture Supernatant is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.|
|Cellular Location||Peroxisome. Mitochondrion|
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Provided below are standard protocols that you may find useful for product applications.
This antibody recognizes a protein of 54kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and β-oxidation of branched-chain fatty acids. AMACR is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands.
1. Xu J et. al. Canc Res. 2000; 60:1677
2. Jiang Z et. al. Human Pathology. 2003; 34(8):792
3. Jiang Z et. al. Am J Surg Pathol. 2001; 25(11):1397
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