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CD45RA (Leucocyte Marker) Antibody - With BSA and Azide

Purified Mouse Monoclonal Antibody

  •  -  CD45RA (Leucocyte Marker) Antibody - With BSA and Azide AH10014
    Formalin-paraffin human spleen stained with CD45RA Ab (158-4D3).
  •  -  CD45RA (Leucocyte Marker) Antibody - With BSA and Azide AH10014
    Surface staining of PBMC using CD45RA Ab (158-4D3) (red), anti mouse IgG FITC secondary and mouse IgG2a isotype control (green).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08575
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a, kappa
Clone Names 158-4D3
Calculated MW 205-220kDa
Additional Information
Gene ID 5788
Other Names Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
Target/Specificity Stimulated human leukocytes
Application Note ELISA : For coating, order Ab without BSA
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 1-2ug/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
Format 0.5 ml at 200ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions CD45RA (Leucocyte Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms CD45
Function Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein Membrane raft. Note=Colocalized with DPP4 in membrane rafts
Research Areas
Citations (0)

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Recognizes a protein of 205kDa-220kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).


1. Oxford University Press, Oxford, p511-515, 1995.
2. Clement LT. J Clin Immunol. 1992, 12(1):1-10.
3. Yamada A, et al. Cell Immunol. 1992, 142(1):114-24.
4. Mahalingam M, et al. Clin Immunol Immunopathol. 1996,81(2):210-214.
5. Jacob MC, et al. Am J Hematol. 1992, 39(1):45-51

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$ 124.00
$ 324.00
$ 454.00
Cat# AH10014
(40 western blots)
Availability: 2-3 days
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