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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >    Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody - With BSA and Azide   

Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody - With BSA and Azide

Purified Mouse Monoclonal Antibody

  •  -  Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody - With BSA and Azide AH10016
    Formalin-fixed, paraffin-embedded human leiomyosarcoma stained with smooth muscle actin MAb (1A4).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P62736
Reactivity Human, Mouse, Rat, Guinea Pig
Predicted Chicken, Cat, Dog, Goat, Pig, Monkey, Rabbit, Sheep
Host Mouse
Clonality Monoclonal
Isotype IgG2a, kappa
Clone Names 1A4; same as asm-1
Calculated MW 42kDa
Additional Information
Gene ID 59
Other Names Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS
Target/Specificity N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH
Application Note ELISA : For coating, order Ab without BSA
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1.0 µg/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 0.5-1 µg/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.).
Format 0.5 ml at 200ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTA2
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Citations (0)

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Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.


1. Skalli O et. al. Journal of Cell Biology, 1986, 103:2787-96.

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$ 124.00
$ 324.00
$ 454.00
Cat# AH10016
(40 western blots)
Availability: 2-3 days
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