Human Nuclear Antigen (HNA) (Human Cell Marker) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||IHC, IF, IP, ICC|
|Calculated MW||70kDa & 80kDa|
|Target/Specificity||Nuclei of human myeloid leukemia biopsy cells|
|Application Note||Immunofluorescence : 0.5-1.0 µg/ml|
Immunoprecipitation : 0.5-1 µg/500ug protein lysate
Immunocytochemistry (Acetone-fixed cells) (0.25-0.5ug/ml for 30 minutes at RT)
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Human Nuclear Antigen (HNA) (Human Cell Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
1. Rosario Sanchez-Pernaute et. al. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease. Brain. 2008; 131(8): 2127–2139.
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