Insulin / IRDN (beta-Cell & Insulinoma Marker) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC, FC, IP, E|
|Other Accession||P01315, P01317|
|Clone Names||2D11-H5; same as INS05|
|Other Names||Insulin, Insulin B chain, Insulin A chain, INS|
|Target/Specificity||Full length (1-84 amino acid) purified pig insulin, conjugated to KLH|
|Application Note||ELISA : For coating, order Ab without BSA|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 1-2ug/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(No special pretreatment is required for staining of formalin/paraffin tissues.).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Insulin / IRDN (beta-Cell & Insulinoma Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.|
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Provided below are standard protocols that you may find useful for product applications.
Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.
1. de la Tour, D., et al. 2001. Mol. Endoc. 15: 476-483.
2. Rajagopal, J., et al. 2003. Science 299: 363.
3. Morisset, J., et al. 2003. J. Histochem. Cytochem. 51: 1501-1513.
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