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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >    Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody - With BSA and Azide   

Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody - With BSA and Azide

Purified Mouse Monoclonal Antibody

  •  -  Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody - With BSA and Azide AH10033
    Formalin-fixed, paraffin-embedded human prostate (10X) stained with Cytokeratin, HMW MAb (34bE12).
  •  -  Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody - With BSA and Azide AH10033
    Formalin-fixed, paraffin-embedded human prostate (20X) stained with Cytokeratin, HMW MAb (34bE12).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04264
Other Accession P13647, P13645, P02533
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1, kappa
Clone Names 34BE12
Calculated MW 67kDa (CK1); 58kDa (CK5); 56.5kDa (CK10); 50kDa (CK14)
Additional Information
Gene ID 3848
Other Names Keratin, type II cytoskeletal 1, 67 kDa cytokeratin, Cytokeratin-1, CK-1, Hair alpha protein, Keratin-1, K1, Type-II keratin Kb1, KRT1, KRTA
Target/Specificity Solubilized keratin extract from human stratum corneum
Application Note Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1.0 µg/ml
Western Blotting : 0.5-1.0 µg/ml
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 min at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
Format 0.5 ml at 200ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT1
Synonyms KRTA
Function May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
Cellular Location Cell membrane. Note=Located on plasma membrane of neuroblastoma NMB7 cells
Tissue Location The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis
Research Areas
Citations (0)

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This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.


1. Moinfar F et. al. Am J Surg Pathol 1999;23(9):1048-58
2. Varma M et. al. Mod Pathol 1999;12(5):472-8

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$ 124.00
$ 324.00
$ 454.00
Cat# AH10033
(40 western blots)
Availability: 2-3 days
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