Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||IHC, IF, FC, IP, E|
|Other Accession||P02533, P19012, P08789, P08727|
|Reactivity||Human, Mouse, Rat|
|Predicted||Chicken, Dog, Monkey, Rabbit|
|Other Names||Keratin, type I cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KRT10, KPP|
|Target/Specificity||Solubilized keratin extract from human stratum corneum|
|Application Note||ELISA : For coating, order Ab without BSA|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western Blotting : 0.5-1.0 µg/ml
Immunohistology (Frozen & formalin-fixed) : 0.5-1.0 µg/ml for 30 min at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Seen in all suprabasal cell layers including stratum corneum|
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Provided below are standard protocols that you may find useful for product applications.
This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50’, 48, 46, 45, and 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
1. Moinfar F et. al. Am J Surg Pathol 1999;23(9):1048-58
2. Varma M et. al. Mod Pathol 1999;12(5):472-8
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