Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC, FC, IP, E|
|Other Accession||P12035, P19013, P13647, P02538, P05787|
|Reactivity||Human, Mouse, Rat|
|Predicted||Chicken, Dog, Monkey, Rabbit|
|Other Names||Keratin, type II cytoskeletal 1, 67 kDa cytokeratin, Cytokeratin-1, CK-1, Hair alpha protein, Keratin-1, K1, Type-II keratin Kb1, KRT1, KRTA|
|Target/Specificity||Solubilized keratin extract from human stratum corneum|
|Application Note||ELISA (For coating, order Ab without BSA)|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western Blotting : 0.5-1.0 µg/ml
Immunohistology (Frozen & formalin-fixed) : 0.5-1.0 µg/ml for 30 min at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.|
|Cellular Location||Cell membrane. Note=Located on plasma membrane of neuroblastoma NMB7 cells|
|Tissue Location||The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis|
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Provided below are standard protocols that you may find useful for product applications.
This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50’, 48, 46, 45, and 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
1. Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8.
2. Tseng SCG et. al. Cell 1982; 30361.
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