|Application ||IHC, IF, FC, E|
|Calculated MW||70kDa (55kDa-Heavy Chain; 15kDa-Light Chain)|
|Other Names||Eosinophil peroxidase, EPO, Eosinophil peroxidase light chain, Eosinophil peroxidase heavy chain, EPX, EPER, EPO, EPP|
|Target/Specificity||Human eosinophils from a patient with hypereosinophilic syndrome|
|Application Note||ELISA (For coating, order Ab without BSA)|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1.0 µg/ml
Immunohistology (Frozen) (0.5-1.0 µg/ml for 30 minutes at RT).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Eosinophil Peroxidase (EPX) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||EPER, EPO, EPP|
|Function||Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis.|
|Cellular Location||Cytoplasmic granule. Note=Cytoplasmic granules of eosinophils|
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Provided below are standard protocols that you may find useful for product applications.
Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).
1. Abbas, A., A.H. Lichtman and J.S. Pober. 1994. Cellular and Molecular Biology, W.B. Saunders Co., Philadelphia, London, Toronto, Tokyo), pp 14-30.
2. Skubitz, K.M., N.P. Christiansen and J.R. Mendiola. 1989. Preparation and characterization of monoclonal antibodies to human neutrophil cathepsin G, lactoferrin, eosinophil peroxidase, and eosinophil major basic protein. J. Leukocyte Biol. 46:109-118.
3. Skubitz, K.M., N.G. Wehner and B.H. Gray. 1988. Preparation and characterization of monoclonal antibody that inhibits human neutrophil elastase activity. J. leukocyte Bio. 44:158-165.
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