EGFR (Epidermal Growth Factor Receptor) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||FC, IP|
|Calculated MW||~170kDa (wild type) and ~145kDa (vIII variant)|
|Other Names||Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1|
|Target/Specificity||Microsomes from A431 cells|
|Application Note||Affinity Purification : For coupling, order Ab without BSA |
Blocks Ligand-induced Activation of EGFR;
Inhibits Proliferation of A431 Cells : Order Ab without sodium azide
Arrests Tumor Growth in vivo : Order Ab without BSA &Azide
Immunoprecipitation : 1-2 µg/500ug protein lysate
Flow Cytometry : 0.5-1ug/million cells.
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||EGFR (Epidermal Growth Factor Receptor) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ERBB, ERBB1, HER1|
|Function||Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Plays a role in enhancing learning and memory performance (By similarity).|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF)|
|Tissue Location||Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.|
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Provided below are standard protocols that you may find useful for product applications.
This antibody reacts with the extracellular domain of EGFR and blocks the EGF/TGFα−induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin.
1. Kudlow JE et. al. Journal of Biological Chemistry, 1986, 261(9):4134-8.
2. Bjorge JD et. al. Journal of Biological Chemistry, 1989, 264(7):4021-7.
3. Bjorge JD et. al. Journal of Biological Chemistry, 1987, 262(14):6615-22.
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