|Application ||WB, IHC, IF, FC, IP|
|Isotype||IgG1, kappa + IgG1, kappa|
|Other Names||Prolactin receptor, PRL-R, PRLR|
|Target/Specificity||Semi-purified human prolactin receptor (B6.2); Recombinant human prolactin receptor (PRLR742)|
|Application Note||Flow Cytometry : 0.5-1ug/million cells|
Immunofluorescence : 0.5-1.0 µg/ml
Western Blotting : 0.25-0.5ug/ml
Immunoprecipitation : 0.5-1 µg/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C.).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Prolactin Receptor (hPRL-Receptor) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This is a receptor for the anterior pituitary hormone prolactin (PRL). Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Expressed in breast, placenta, kidney, liver and pancreas.|
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Provided below are standard protocols that you may find useful for product applications.
It recognizes a protein of 70kDa, identified as prolactin receptor. Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor), which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription.
1. Br J Cancer. 2011;104(10):1641-8.
2. Breast Cancer Res. 2008;10(4):R68.
3. Histopathology. 2008;53(1):56-61.
4. Mod Pathol. 2010;23(7):961-71.
5. Breast Cancer Res Treat. 2011;128(1):31-40.
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