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>   home   >   Products   >   Primary Antibodies   >    Myeloid Cell Marker (Macrophage / Granulocyte Marker) Antibody - With BSA and Azide   

Myeloid Cell Marker (Macrophage / Granulocyte Marker) Antibody - With BSA and Azide

Purified Mouse Monoclonal Antibody

     
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC, IF, FC
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1, kappa
Clone Names BM-1
Calculated MW 183kDa
Additional Information
Target/Specificity Human peripheral blood mononuclear cells
Application Note Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(No special pretreatment is required for staining of formalin/paraffin tissues.).
Format 0.5 ml at 200ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions Myeloid Cell Marker (Macrophage / Granulocyte Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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Background

Recognizes 183kDa protein with DNA-binding characteristics, which is identified as a myeloid specific antigen. BM-1 reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. BM-1 and BM-2 antibodies react with early precursor and mature forms of human myeloid cells. BM-1 MAb is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of this antigen is not clear, although it has been proposed that BM-1 may play a role in the differentiation of myeloid cells.

References

1. Epstein AL; Samoszuk M; Stathopoulos E; Naeve GS; Clevenger CV; Weil S; Marder RJ. Immunohistochemical characterization of a 183 KD myeloid-specific-DNA-binding protein in B5 fixed, paraffin-embedded tissues, and bone marrow aspirates by monoclonal antibody BM-1. Blood, 1987, 70(4):1124-30.

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$ 124.00
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Cat# AH10082
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