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CD45RB Antibody [Clone BRA-11; same as BRA-11G]
Purified Mouse Monoclonal Antibody
![- CD45RB Antibody [Clone BRA-11; same as BRA-11G] AH10087-50](/assets/uploads/products/201408/AH10087_1.jpg)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC |
|---|---|
| Primary Accession | P08575 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Clone Names | BRA-11; same as BRA-11G |
| Calculated MW | 180-220kDa |
| Gene ID | 5788 |
|---|---|
| Other Names | Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45 |
| Target/Specificity | Non-T, non-B CALLA positive ALL cell line REH (Leucocyte Workshop IV and V) |
| Application Note | Flow Cytometry 2.5ul (0.5ug) per test per one million cells. |
| Format | 0.5 ml at 100ug/ml; Conjugated to AF488 |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | CD45RB Antibody [Clone BRA-11; same as BRA-11G] is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PTPRC (HGNC:9666) |
|---|---|
| Synonyms | CD45 |
| Function | Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein. Membrane raft Note=Colocalized with DPP4 in membrane rafts |
| Tissue Location | Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes Isoform 8: Not detected in thymocytes. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
References
1. Sedlak et al. Neoplasma 1989: 643, 1989.
2. Sutherland et.al Int. Immunol., 14(8): 953 – 962 (August 1, 2002)
3. Lim et.al Int. Immunol., 18(2):291-300 (2006)
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