Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||IHC, IF, FC|
|Reactivity||Human, Mouse, Rat|
|Predicted||Human, Mouse, Rat|
|Calculated MW||307 Dalton|
|Target/Specificity||Bromodeoxyuridine (BrdU) conjugated to KLH|
|Application Note||Flow Cytometry : 0.5-1ug/million cells|
Immunofluorescence : 0.5-1.0 µg/ml
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(Use Ab at 1mg/ml for 30 minute at RT)
[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC (Cat. #AP-9008)].
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
1. Welberg JW et. al. Journal of Clinical Pathology, 1990, 43(6):453-6.
2. Williams LS et. al. Cytometry, 1990, 11(4):490-7.
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