GM-CSF (Granulocyte/Macrophage - Colony Stimulating Factor) Antibody - With BSA and Azide
Rat Monoclonal Antibody
|Application ||WB, IHC, IF, FC, IP, E|
|Other Names||Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF, Molgramostin, Sargramostim, CSF2, GMCSF|
|Target/Specificity||Recombinant human GM-CSF protein|
|Application Note||ELISA : For coating, order Ab without BSA|
Neutralization Studies : Order Ab without Azide
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1.0 µg/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 0.5-1 µg/500ug protein lysate
Immunohistology (Frozen) : 0.5-1.0 µg/ml for 30 minutes at RT.
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||GM-CSF (Granulocyte/Macrophage - Colony Stimulating Factor) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.|
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Provided below are standard protocols that you may find useful for product applications.
Granulocyte/macrophage - Colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.
1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
2. Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133.
3. Bacchetta R, et al. 1990. J. Immunol. 144:902.
4. Kita H, et al. 1991. J. Exp. Med. 174:745.
5. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
6. Andersson J, et al. 1994. Immunology 83:16.
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