Cytokeratin, Multi (Epithelial Marker) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC, IF, FC, IP|
|Other Accession||P13647, P02538, P05787, P13645, P13646, P05783|
|Reactivity||Human, Mouse, Rat, Guinea Pig|
|Calculated MW||Multiple KDa|
|Other Names||Keratin, type II cytoskeletal 4, Cytokeratin-4, CK-4, Keratin-4, K4, Type-II keratin Kb4, KRT4, CYK4|
|Target/Specificity||Keratin-enriched preparation from cultured human A431|
|Application Note||Flow Cytometry : 0.5-1ug/million cells|
Immunofluorescence : 0.5-1.0 µg/ml
Western Blotting : 0.5-1.0 µg/ml
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 min at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Cytokeratin, Multi (Epithelial Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer|
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Provided below are standard protocols that you may find useful for product applications.
This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
1. Bartek J et. al. J Pathol, 1991, 164(3):215-24.
2. Lane EB; Alexander CM. Seminars in Cancer Biology, 1990, 1:165-79.
3. Bartkova J; et al. Neoplasma, 1991, 38:439-46.
4. Kasper M. Histochemistry, 1991, 95(6):613-20.
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