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Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, FC, IP, E |
|---|---|
| Reactivity | Human, Rat |
| Predicted | Dog |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Clone Names | CA72/733 |
| Calculated MW | 220kDa |
| Target/Specificity | Recombinant human TAG-72 protein |
|---|---|
| Application Note | ELISA : For coating, order Ab without BSA Flow Cytometry : 0.5-1ug/million cells Immunofluorescence : 1-2ug/ml Western Blotting : 0.5-1.0 µg/ml Immunoprecipitation : 1-2ug/500ug protein lysate Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT (No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues). |
| Format | 0.5 ml at 200ug/ml with BSA and azide |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
References
1. Lottich SC et. al. Breast Cancer Research and Treatment, 1985, 6(1):49-56.
2. Thor A et. al. Cancer Research, 1986, 46(6):3118-24.
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