Carcinoembryonic Antigen (CEA) / CD66 Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC, IF, FC, IP|
|Isotype||IgG2a (COL-1) + IgG1 (CEA31 & C66/261)|
|Clone Names||COL-1 + CEA31 + C66/261|
|Other Names||Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen, CEA, Meconium antigen 100, CD66e, CEACAM5, CEA|
|Target/Specificity||Human colon carcinoma extract (COL-1 & CEA31); CEA recombinant protein (C66/261)|
|Application Note||Flow Cytometry : 0.5-1ug/million cells|
Immunofluorescence : 1-2ug/ml
Immunoprecipitation : 1-2ug/500ug protein
Western Blotting : 0.25-0.5ug/ml
Immunohistology (Frozen & formalin-fixed) : 1-2ug/ml for 30 min at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Carcinoembryonic Antigen (CEA) / CD66 Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor|
|Tissue Location||Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon|
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Provided below are standard protocols that you may find useful for product applications.
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb reacts with nonspecific cross-reacting antigen (NCA) and shows a cross-reaction with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
1. Muraro R, et. al. Cancer Research, 1985, 45:5769-80.
2. Siler K, et. al. Biotechnology Therapeutics, 1993, 4(3-4):163-81.
3. Robbins PF, et. al. International Journal of Cancer, 1993, 53(6):892-7.
4. Shi ZR, et. al. Journal of Histochemistry and Cytochemistry, 1994, 42(9):1215-9.
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