|Application ||WB, IP|
|Other Names||T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, CD5, CD5, LEU1|
|Target/Specificity||Stimulated human leukocytes|
|Application Note||ELISA (For coating, order Ab without BSA)|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1.0 µg/ml
Functional Studies : Order Ab without BSA & Azide
Immunoprecipitation : 0.5-1 µg/500ug protein lysate
Immunohistology (Frozen) (0.5-1.0 µg/ml for 30 minutes at RT).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD5 (Mantle Cell Lymphoma Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May act as a receptor in regulating T-cell proliferation.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Recognizes a 67kDa transmembrane protein, which is identified as CD5 (HLDA I; WS Code T 29. HLDA III; WS Code T 530). The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms.
1. Leukocyte Typing III., McMichael A. J. et al. (Eds.), Oxford University Press (1987).
2. Arrizabalaga P, Mirapeix E, Darnell A, Torras A, Revert L: Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis. Nephron. 1989;53(1):41-9.
3. Alberola-Ila J, Places L, Cantrell DA, Vives J, Lozano F: Intracellular events involved in CD5-induced human T cell activation and proliferation. J Immunol. 1992;148(5):1287-93.
4. Guarne A, Bravo J, Calvo J, Lozano F, Vives J, Fita I: Conformation of the hypervariable region L3 without the key proline residue. Protein Sci. 1996;5(1):167-9.
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