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Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF, FC, IP, E |
|---|---|
| Primary Accession | P01871 |
| Other Accession | P20769 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Clone Names | DA4-4; same as SA-DA4 or HB57 |
| Calculated MW | 75kDa |
| Other Names | Ig mu chain C region, IGHM |
|---|---|
| Target/Specificity | Heavy chain of human IgM |
| Application Note | ELISA : For coating, order Ab without BSA Flow Cytometry : 0.5-1ug/million cells Immunofluorescence : 0.5-1.0 µg/ml Western Blotting : 0.5-1.0 µg/ml Immunoprecipitation : 0.5-1 µg/500ug protein lysate Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). |
| Format | 0.5 ml at 200ug/ml with BSA and azide |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | IGHM |
|---|---|
| Function | IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane- bound form is found in the majority of normal B-cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30% of the total serum immunoglobulins where it is found almost exclusively as a homopentamer. After the antigen binds to the B-cell receptor, the secreted form is secreted in large amounts. |
| Cellular Location | Isoform 1: Secreted. Note=During differentiation, B-lymphocytes switch from expression of membrane- bound IgM to secretion of IgM |
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Provided below are standard protocols that you may find useful for product applications.
Background
Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
References
1. Maruyama S, et. al. Activation of human B cells and inhibition of their terminal differentiation by monoclonal anti-mu antibodies. Journal of Immunology 1985; 135(1):192-9.
2. Rudich SM, et. al. Human B cell activation. Evidence for diverse signals provided by various monoclonal anti-IgM antibodies. Journal of Experimental Medicine, 1985; 162(4):1236-55.
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