|Application ||WB, IHC, FC, IP, E|
|Calculated MW||85-95kDa (monomer); 190kDa (dimer)|
|Other Names||Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, CD71, Transferrin receptor protein 1, serum form, sTfR, TFRC|
|Target/Specificity||KG1 acute myeloid leukemia cell line|
|Application Note||ELISA : For coating, order Ab without BSA |
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western Blotting : 0.25-0.5ug/ml
Immunohistology (Frozen & formalin-fixed) : 1-2ug/ml for 30 min at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD71 / Transferrin Receptor (TFRC) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).|
|Cellular Location||Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV|
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Provided below are standard protocols that you may find useful for product applications.
It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV). This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
1. Ben-David, D. et al. 2010. J Tissue Eng Regen Med. 4: 30-37.
2. Srouji, S. et al. 2009. Calcif. Tissue Int. 84: 138-145.
3. Kim, DS. et al. 2007. J. Dermatol. Sci. 46: 65-68.
4. Fritzius, T. et al. 2006. Biochem J. 399(1): 9-20.
5. Harigae, H. et al. 2006. Int. J. Hematol. 84: 38-42.
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