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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >    CD43 (T-Cell Marker) Antibody - With BSA and Azide   

CD43 (T-Cell Marker) Antibody - With BSA and Azide

Purified Mouse Monoclonal Antibody

  •  -  CD43 (T-Cell Marker) Antibody - With BSA and Azide AH10150
    Formalin-paraffin spleen stained with CD43 Ab (DF-T1).
  •  -  CD43 (T-Cell Marker) Antibody - With BSA and Azide AH10150
    CD43 expression by lymphocyte gated population of PBMC: PBMC were stained either with purified CD43 Ab (DF-T1) or isotype control.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P16150
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1, kappa
Clone Names DF-T1
Calculated MW 95, 115, or 135kDa
Additional Information
Gene ID 6693
Other Names Leukosialin, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, CD43, SPN, CD43
Target/Specificity Myeloblastic KG1 cells
Application Note ELISA : For coating, order Ab without BSA
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 1-2ug/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
Format 0.5 ml at 200ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions CD43 (T-Cell Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPN
Synonyms CD43
Function One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas
Research Areas
Citations (0)

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It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.


1. Stross WP, et. al. Journal of Clinical Pathology, 1989, 42(9):953-61.

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$ 124.00
$ 324.00
$ 454.00
Cat# AH10150
(40 western blots)
Availability: 2-3 days
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