Melanoma Marker (MART-1 + gp100) Antibody [Clone DT101+ BC199 + HMB45]
Purified Mouse Monoclonal Antibody
|Isotype||IgG2b (DT101& BC199) + IgG1 (HMB45)|
|Clone Names||DT101+ BC199 + HMB45|
|Calculated MW||20-22kDa (doublet) (MART-1); 90-100kDa (gp100)|
|Other Names||Melanoma antigen recognized by T-cells 1, MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A, MLANA, MART1|
|Target/Specificity||Recombinant hMART-1 protein (DT101& BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45)|
|Application Note||Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT|
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Melanoma Marker (MART-1 + gp100) Antibody [Clone DT101+ BC199 + HMB45] is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation|
|Tissue Location||Expression is restricted to melanoma and melanocyte cell lines and retina|
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Provided below are standard protocols that you may find useful for product applications.
This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
1. Chen Y-T, et. al. Proc Natl Acad Sci, USA, 1996, 93:5915-19.
2. Chen Y-T, et. al. Proc. Natl. Acad. Sci. USA, 1995, 92:8125-8129.
3. Gown AM, et. al. American Journal of Pathology, 1986, 123(2):195-203.
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