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Insulin / IRDN (beta-Cell & Insulinoma Marker) Antibody - With BSA and Azide

Purified Mouse Monoclonal Antibody

  •  -  Insulin / IRDN (beta-Cell & Insulinoma Marker) Antibody - With BSA and Azide AH10163
    Formalin-fixed, paraffin-embedded human pancreas stained with Insulin MAb (E2-E3). Note cytoplasmic staining of cells.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01308
Other Accession P01315, P01317
Reactivity Human, Rat
Predicted Pig, Rabbit
Host Mouse
Clonality Monoclonal
Isotype IgG1, kappa
Clone Names E2-E3; same as INS04
Calculated MW 6kDa
Additional Information
Gene ID 3630
Other Names Insulin, Insulin B chain, Insulin A chain, INS
Target/Specificity Full length (1-84 amino acid) purified pig insulin, conjugated to KLH
Application Note ELISA : For coating, order Ab without BSA
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 1-2ug/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(No special pretreatment is required for staining of formalin/paraffin tissues.).
Format 0.5 ml at 200ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions Insulin / IRDN (beta-Cell & Insulinoma Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name INS
Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Cellular Location Secreted.
Research Areas
Citations (0)

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Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.


1. de la Tour, D., et al. 2001. Mol. Endoc. 15: 476-483.
2. Rajagopal, J., et al. 2003. Science 299: 363.
3. Morisset, J., et al. 2003. J. Histochem. Cytochem. 51: 1501-1513.

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$ 124.00
$ 324.00
$ 454.00
Cat# AH10163
(40 western blots)
Availability: 2-3 days
Bulk Size
Seasonal Special on Bulk Order
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