|Application ||WB, IF, FC, IP, E|
|Other Names||Cyclin-A2, Cyclin-A, CCNA2, CCN1, CCNA|
|Target/Specificity||Full length bovine Cyclin A protein|
|Application Note||ELISA : For coating, order antibody without BSA|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Immunoprecipitation : 1-2ug/500ug protein
Western Blotting : 0.5-1.0 µg/ml
Immunohistology (Frozen & formalin-fixed) : 1-2ug/ml for 30 min at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Cyclin A2 (S- & G2-phase Cyclin) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.|
|Cellular Location||Nucleus. Cytoplasm. Note=Cytoplasmic when associated with SCAPER|
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Provided below are standard protocols that you may find useful for product applications.
It recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk’s) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk’s. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.
1. Adamczewski JP; Gannon JV; Hunt T. Simian virus 40 large T antigen associates with cyclin A and p33cdk2. Journal of Virology, 1993, 67(11):6551-7.
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