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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >    HSP27 (Heat Shock Protein 27) Antibody - With BSA and Azide   

HSP27 (Heat Shock Protein 27) Antibody - With BSA and Azide

Purified Mouse Monoclonal Antibody

  •  -  HSP27 (Heat Shock Protein 27) Antibody - With BSA and Azide AH10178
    Formalin-fixed, paraffin-embedded human breast carcinoma (10X) stained with HSP27 MAb (G3.1)
  •  -  HSP27 (Heat Shock Protein 27) Antibody - With BSA and Azide AH10178
    Formalin-paraffin breast carcinoma (20X) stained with HSP27 Ab (G3.1)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04792
Other Accession P14602, P42930
Reactivity Human, Mouse, Rat
Predicted Chicken, Sheep
Host Mouse
Clonality Monoclonal
Isotype IgG1, kappa
Clone Names G3.1
Calculated MW 27kDa
Additional Information
Gene ID 3315
Other Names Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28
Target/Specificity Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Application Note ELISA (For coating, order Ab without BSA)
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1.0 µg/ml
Western Blotting : 0.25-0.5ug/ml
Immunoprecipitation : 0.5-1 µg/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
Format 0.5 ml at 200ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions HSP27 (Heat Shock Protein 27) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSPB1
Synonyms HSP27, HSP28
Function Involved in stress resistance and actin organization.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles
Tissue Location Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle
Research Areas
Citations (0)

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It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced “p29” and “24K” protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.


1. Edwards DP et. al. Biochem Biophys Research Commun, 93:804-812, 1980.
2. Ciocca DR et. al. Breast Cancer Research and Treatment, 20:33-42, 1991.

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$ 124.00
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$ 454.00
Cat# AH10178
(40 western blots)
Availability: 2-3 days
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