GFAP (Astrocyte & Neural Stem Cell Marker) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC, IF, FC, IP, E|
|Other Accession||P03995, P47819|
|Reactivity||Human, Mouse, Rat|
|Predicted||Chicken, Pig, Rabbit|
|Other Names||Glial fibrillary acidic protein, GFAP, GFAP|
|Target/Specificity||GFAP isolated from pig spinal cord|
|Application Note||ELISA : For coating, order Ab without BSA|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 1-2ug/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||GFAP (Astrocyte & Neural Stem Cell Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.|
|Cellular Location||Cytoplasm. Note=Associated with intermediate filaments|
|Tissue Location||Expressed in cells lacking fibronectin.|
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Provided below are standard protocols that you may find useful for product applications.
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
1. Herpers MJ et. Al. 1986, Acta Neuropathol, 70:333-339.
2. Van Muijen GN et. al. 1987, Lab Invest, 57:359-369.
3. Debus E, et. al. 1983, Differentiation, 25(2):193-203.
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