|Application ||WB, IHC, IF, FC, IP|
|Other Names||Epithelial cell adhesion molecule, Ep-CAM, Epithelial glycoprotein 314, EGP314, Protein D57A, Tumor-associated calcium signal transducer 1, CD326, Epcam, Tacstd1|
|Target/Specificity||Rat small intestine cells|
|Application Note||Flow Cytometry : 5-10ul/million cells|
Immunofluorescence : 1:50-1:100
Western Blotting :1:100-1:200
Immunoprecipitation : 5-10ul/500ug protein lysate
Immunohistology (Frozen & formalin-fixed) (1:50-1:100 for 30 min at RT)
(Formalin/paraffin tissues REQUIRE digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C).
|Format||0.5 ml of Bioreactor Concentrate with azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||EpCAM (Rat) Antibody [Clone GZ-20] is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).|
|Cellular Location||Lateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Note=Colocalizes with CLDN7 at the lateral cell membrane and tight junction|
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Provided below are standard protocols that you may find useful for product applications.
Epitope of GZ-1 and GZ-20 monoclonal antibodies are different. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.
1. Litvinov SV et. al. J Cell Biol, 1994, 125:437-46.
2. Tsubura A et. al. J Cut Pathol, 1992, 19:73-9.
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