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>   home   >   Products   >   Primary Antibodies   >   EpCAM (Rat) Antibody [Clone GZ-20]   

EpCAM (Rat) Antibody [Clone GZ-20]

Purified Mouse Monoclonal Antibody

  •  - EpCAM  (Rat) Antibody [Clone GZ-20] AH10188
    Western blot analysis of rat colon probed with Ep-CAM Ab (GZ-20).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O55159
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names GZ-20
Calculated MW 40-43kDa
Additional Information
Gene ID 171577
Other Names Epithelial cell adhesion molecule, Ep-CAM, Epithelial glycoprotein 314, EGP314, Protein D57A, Tumor-associated calcium signal transducer 1, CD326, Epcam, Tacstd1
Target/Specificity Rat small intestine cells
Application Note Flow Cytometry : 5-10ul/million cells
Immunofluorescence : 1:50-1:100
Western Blotting :1:100-1:200
Immunoprecipitation : 5-10ul/500ug protein lysate
Immunohistology (Frozen & formalin-fixed) (1:50-1:100 for 30 min at RT)
(Formalin/paraffin tissues REQUIRE digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C).
Format 0.5 ml of Bioreactor Concentrate with azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
PrecautionsEpCAM (Rat) Antibody [Clone GZ-20] is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Epcam
Synonyms Tacstd1
Function May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).
Cellular Location Lateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Note=Colocalizes with CLDN7 at the lateral cell membrane and tight junction
Citations (0)

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Epitope of GZ-1 and GZ-20 monoclonal antibodies are different. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.


1. Litvinov SV et. al. J Cell Biol, 1994, 125:437-46.
2. Tsubura A et. al. J Cut Pathol, 1992, 19:73-9.

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$ 295.00
Cat# AH10188
(40 western blots)
Availability: 2-3 days
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