HCG-beta (Pregnancy & Choriocarcinoma Marker) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC|
|Isotype||IgG1 + IgG1|
|Clone Names||HCGb/54+ HCGb/459|
|Other Names||Choriogonadotropin subunit beta, CG-beta, Chorionic gonadotrophin chain beta, CGB, CGB3|
|Target/Specificity||Recombinant hCG beta protein (HCGb/54 and HCGb/459)|
|Application Note||Western Blotting : 0.5-1.0 µg/ml|
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||HCG-beta (Pregnancy & Choriocarcinoma Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
This MAb reacts with a protein of 22kDa, identified as beta sub-unit of HCG. It does not cross react with the alpha sub-unit. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the α and β subunits. The α subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). hCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior.
1. Cocquebert M et. al. Am J Physiol Endocrinol Metab. 2012;303(8):E950-8.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.