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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CD99 Antibody [Clone HO36-1.1]   

CD99 Antibody [Clone HO36-1.1]

Purified Mouse Monoclonal Antibody

  •  - CD99 Antibody [Clone HO36-1.1] AH10206
    Formalin-fixed, paraffin-embedded human Ewing’s sarcoma stained with CD99 MAb (HO36-1.1).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14209
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Isotype IgM, kappa
Clone Names HO36-1.1
Calculated MW 27-32kDa
Additional Information
Gene ID 4267
Other Names CD99 antigen, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2, CD99, CD99, MIC2, MIC2X, MIC2Y
Target/Specificity Purified E-rosette forming cells from human peripheral blood lymphocytes
Application Note Immunofluorescence : 1:50-1:100
Immunohistology (Frozen & Formalin-fixed) (1:50-1:100 for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
Format 0.5 ml of Bioreactor Concentrate with azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
PrecautionsCD99 Antibody [Clone HO36-1.1] is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD99
Synonyms MIC2, MIC2X, MIC2Y
Function Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Citations (0)

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Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing’s sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.


1. Sandrin MS, et. al. Immunogenetics, 1992, 35(4):283-5.

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$ 295.00
Cat# AH10206
(40 western blots)
Availability: 2-3 days
Bulk Size
Seasonal Special on Bulk Order
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