|Application ||IHC, IF|
|Other Names||CD99 antigen, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2, CD99, CD99, MIC2, MIC2X, MIC2Y|
|Target/Specificity||Purified E-rosette forming cells from human peripheral blood lymphocytes|
|Application Note||Immunofluorescence : 1:50-1:100|
Immunohistology (Frozen & Formalin-fixed) (1:50-1:100 for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml of Bioreactor Concentrate with azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD99 Antibody [Clone HO36-1.1] is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||MIC2, MIC2X, MIC2Y|
|Function||Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).|
|Cellular Location||Membrane; Single-pass type I membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing’s sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
1. Sandrin MS, et. al. Immunogenetics, 1992, 35(4):283-5.
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