Hepatocyte Specific Antigen (Hepatocellular Marker) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC, IF, FC, E|
|Target/Specificity||SK-H1A9-2 human hepatocellular carcinoma cells|
|Application Note||Immunofluorescence : 0.5-1.0 µg/ml|
Immuno-cytochemistry (Acetone-fixed cells) (0.5-1.0 µg/ml for 30 minutes at RT)
Immunohistology (Frozen) (0.5-1.0 µg/ml for 30 minutes at RT).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Hepatocyte Specific Antigen (Hepatocellular Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
MAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this MAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.
1. Wennerberg AE et. al. Am J Pathol 1993;143:1050-4.
2. Ramos-Vara, J.A., et al. Histochem 2002; J. 34: 397-401.
3. Fan, Z., et al. Mod. Pathol 2003; 16: 137-144, 2003.
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