|Application ||WB, IHC, IF, FC, E|
|Reactivity||Human, Mouse, Rat|
|Predicted||Human, Mouse, Rat|
|Calculated MW||244 Dalton|
|Target/Specificity||Biotinylated sheep immunoglobulin|
|Application Note||Confocal Microscopy; ELISA;|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1.0 µg/ml
In situ Nucleic Acid Hybridization; Western Blotting;
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Biotin (Vitamin B7 or Vitamin H) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
1. Uccellini MB, et al. 2008. J. Immunol. 181:5875.
2. Green NM, et al. 2012. J Biol Chem. 287:39789
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