|Application ||WB, IHC, IF, FC, IP|
|Isotype||IgG1, kappa + IgG1, kappa|
|Clone Names||K8.8 + DC10|
|Calculated MW||52.5kDa (CK8); 45kDa (CK18)|
|Other Names||Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8|
|Target/Specificity||Keratin preparation from a human carcinoma (K8.8); PMC-42 human breast carcinoma cells (DC10)|
|Application Note||Flow Cytometry : 0.5-1ug/million cells|
Immunofluorescence : 1-2ug/ml
Immunoprecipitation : 1-2ug/500ug protein
Western Blotting : 0.5-1.0 µg/ml
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 min at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Cytokeratin 8/18 Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.|
|Cellular Location||Cytoplasm. Nucleus, nucleoplasm Nucleus matrix|
|Tissue Location||Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.|
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Provided below are standard protocols that you may find useful for product applications.
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
1. Angus B et. al. J Path, 153:377-384, 1987.
2. Angus B et. al. J Path, 155:71-75, 1988
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