|Application ||WB, IHC, IF, FC|
|Clone Names||Leu-M1 (same as MMA|
|Other Names||Alpha-(1, 3)-fucosyltransferase 4, 241-, ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Fuc-TIV, FucT-IV, Galactoside 3-L-fucosyltransferase, FUT4, ELFT, FCT3A|
|Target/Specificity||U937 histiocytic cell line|
|Application Note||Flow Cytometry : 5-10ul/million cells|
Immunofluorescence : 1:50-1:100
Western Blotting :1:100-1:200
Immunohistology (formalin-fixed) (1:50-1:100 for 30 min at Room Temp)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml of Bioreactor Concentrate with azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD15 Antibody [Clone Leu-M1 (same as MMA] is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.|
|Cellular Location||Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi|
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Provided below are standard protocols that you may find useful for product applications.
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin’s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
1. Hanjan SN et. al. Clinical Immunology & Immunopathology, 1982;23(2):172-88.
2. Hsu et. al. Amer J Clin Pathol 82: 29, 1984.
3. Pinkus et. al. Am J Pathol 119: 244, 1985.
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