|Application ||WB, IHC, FC|
|Calculated MW||46605 Da|
|Other Names||Beta-galactoside alpha-2, 6-sialyltransferase 1, Alpha 2, 6-ST 1, B-cell antigen CD75, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, 6-sialyltransferase 1, ST6Gal I, ST6GalI, Sialyltransferase 1, ST6GAL1, SIAT1|
|Target/Specificity||Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.|
|Application Note||Flow Cytometry : 5-10ul/million cells|
Immunofluorescence : 1:25-1:50
Immunohistology (Frozen & formalin-fixed) (1:50-1:100; 30 min RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml of Bioreactor Concentrate with azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD75 Antibody [Clone LN1] is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transfers sialic acid from CMP-sialic acid to galactose- containing acceptor substrates.|
|Cellular Location||Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note=Membrane- bound form in trans cisternae of Golgi. Secreted into the body fluid|
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Provided below are standard protocols that you may find useful for product applications.
Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This MAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin’s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.
1. Epstein AL et. al. J of Immunology 133: 1028-1036, 1984.
2. Marder RJ et. al. Lab Invest 52: 497-504, 1985.
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