|Application ||IHC, IF, FC, IP, E|
|Calculated MW||95kDa & 170kDa|
|Other Names||Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, ITGAM, CD11B, CR3A|
|Target/Specificity||B10 mouse spleen cells enriched for T lymphocytes|
|Application Note||ELISA (For coating, order Ab without BSA)|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1.0 µg/ml
Functional Studies : Order Ab without BSA & Azide
Immunoprecipitation : 0.5-1 µg/500ug protein lysate
Immunohistology (Frozen) (0.5-1.0 µg/ml for 30 minutes at RT).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD11b / MAC-1 (Microglial Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Predominantly expressed in monocytes and granulocytes|
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Provided below are standard protocols that you may find useful for product applications.
CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin åM combines with the Integrin ∫2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin åM/∫2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.
1. Springer T, et al. 1978. Eur. J. Immunol. 8:539.
2. Ault K and Springer T. 1981. J. Immunol. 126:359.
3. Springer TA, et al. 1982. Immunol. Rev. 68:171.
4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766.
5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112.
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