Insulin-like Growth Factor-1 (IGF-1) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC, IF, FC, IP, E|
|Other Accession||P05017, P08025|
|Reactivity||Human, Mouse, Rat, Rabbit|
|Other Names||Insulin-like growth factor I, IGF-I, Mechano growth factor, MGF, Somatomedin-C, IGF1, IBP1|
|Target/Specificity||Purified human IGF-1 protein|
|Application Note||ELISA : For coating, purchase antibody without BSA |
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Immunoprecipitation : 1-2ug/500ug protein
Western Blotting : 0.5-1.0 µg/ml
Immunohistology (Formalin-fixed) : 1-2ug/ml for 30 min at RT
In-vitro Neutralization of Biological Activity of IGF-1 (Order Ab without Azide).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Insulin-like Growth Factor-1 (IGF-1) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation.|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.
1. Rotwein p, et. al. (1986) J. Biol. Chem. 261: 4828-4832.
2. Sandberg-Nordqvist AC, et. al. (1993) Cancer Res. 53: 2475-2478.
3. Zheng WH, et. al. (2000) J. Neural.Transm. Suppl. 2000: 261-272.
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