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MART-1 / Melan-A / MLANA (Melanoma Marker) Antibody - With BSA and Azide

Purified Mouse Monoclonal Antibody

  •  -  MART-1 / Melan-A / MLANA (Melanoma Marker) Antibody - With BSA and Azide AH10265
    Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.
  •  -  MART-1 / Melan-A / MLANA (Melanoma Marker) Antibody - With BSA and Azide AH10265
    Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16655
Reactivity Human
Predicted Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b, kappa
Clone Names M2-7C10
Calculated MW 20-22kDa (doublet)
Additional Information
Gene ID 2315
Other Names Melanoma antigen recognized by T-cells 1, MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A, MLANA, MART1
Target/Specificity Recombinant hMART-1 protein
Application Note ELISA : For coating, order Ab without BSA
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 1-2ug/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
Format 0.5 ml at 200ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions MART-1 / Melan-A / MLANA (Melanoma Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MART1
Function Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Cellular Location Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation
Tissue Location Expression is restricted to melanoma and melanocyte cell lines and retina
Research Areas
Citations (0)

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This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.


1. Kawakami Y, et. al. Journal of Immunological Methods, 1997, 202(1):13-25.
2. Marincola FM, et. al. Journal of Immunotherapy with Emphasis on Tumor Immunology, 1996, 19(3):192-205.

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$ 124.00
$ 324.00
$ 454.00
Cat# AH10265
(40 western blots)
Availability: 2-3 days
Bulk Size
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