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Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC |
|---|---|
| Primary Accession | Q16655 |
| Other Accession | P14679, P40967 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b (M2-7C10 & M2-9E3) + IgG2a (T311) + IgG1 (HMB45) |
| Clone Names | M2-7C10 + M2-9E3 + T311 + HMB45 |
| Calculated MW | 20-22kDa (doublet) (MART); 70-80kDa (Tyrosinase); 90-100kDa (gp100) |
| Gene ID | 2315 |
|---|---|
| Other Names | Melanoma antigen recognized by T-cells 1, MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A, MLANA, MART1 |
| Target/Specificity | Recombinant hMART-1 protein (M2-7C10; M2-9E3); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45) |
| Application Note | Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). |
| Format | 0.5 ml at 200ug/ml with BSA and azide |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Melanoma Marker (MART-1 + gp100 + Tyrosinase) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MLANA |
|---|---|
| Synonyms | MART1 |
| Function | Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. |
| Cellular Location | Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation |
| Tissue Location | Expression is restricted to melanoma and melanocyte cell lines and retina |
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Provided below are standard protocols that you may find useful for product applications.
Background
This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
References
1. Chen Y-T, et. al. Proc Natl Acad Sci, USA, 1996, 93:5915-19.
2. Chen Y-T, et. al. Proc. Natl. Acad. Sci. USA, 1995, 92:8125-8129.
3. Gown AM, et. al. American Journal of Pathology, 1986, 123(2):195-203.
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