TGF-alpha (Transforming Growth Factor alpha) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC, FC, IP, E|
|Reactivity||Human, Mouse, Rat|
|Other Names||Protransforming growth factor alpha, Transforming growth factor alpha, TGF-alpha, EGF-like TGF, ETGF, TGF type 1, TGFA|
|Target/Specificity||A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGFα.|
|Application Note||ELISA : For coating, order Ab without BSA|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Immunoprecipitation : 1-2ug/500ug protein
Western Blotting : 0.5-1.0 µg/ml
Immunohistology (Frozen) : 1-2ug/ml for 30 min at RT.
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||TGF-alpha (Transforming Growth Factor alpha) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.|
|Cellular Location||Transforming growth factor alpha: Secreted, extracellular space|
|Tissue Location||Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines|
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Provided below are standard protocols that you may find useful for product applications.
This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGFα (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
1. Kobrin MS et. al. J Bio Chem, 1986, 261:14414-9.
2. Kudlow JE et. al. Endocrinology, 1987, 121(4):1577-9.
3. Kobrin MS et. al. Endocrinology, 1987, 121(4):1412-6.
4. Kudlow JE et. al. J Bio Chem, 1989, 264(7):3880-3
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