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CD3 epsilon Antibody

Purified Rabbit Polyclonal Antibody

  •  - CD3 epsilon Antibody  AH10290
    Western blot of CD3e in human Jurkat cells 1) absence and 2) presence of immunizing peptide, 3) on mouse thymus probed with CD3e Rabbit PAb.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P07766
Reactivity Human, Mouse, Rat, Guinea Pig, Hamster
Predicted Mouse, Chicken, Dog, C.Elegans, Horse, Pig, Monkey, Rabbit, Sheep
Host Rabbit
Clonality Polyclonal
Isotype IgG
Clone Names N/A
Calculated MW 23kDa
Additional Information
Gene ID 916
Other Names T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
Target/Specificity A synthetic peptide aa156-168 (ERPPPVPNPDYEP) from human CD3e
Application Note Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 1-2ug/500ug protein lysate.
Format 0.5 ml at 200ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
PrecautionsCD3 epsilon Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD3E
Synonyms T3E
Function The CD3 complex mediates signal transduction.
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Citations (0)

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Recognizes the -chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.


1. Cibull ML et. al. Histopathology, 1989, 15(6):599-605.
2. Mason DY et. al. Journal of Clinical Pathology, 1989, 42(11):1194-200.

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$ 295.00
Cat# AH10290
(40 western blots)
Availability: 2-3 days
Bulk Size
Seasonal Special on Bulk Order
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