|Application ||WB, IF, FC, IP|
|Reactivity||Human, Mouse, Rat, Guinea Pig, Hamster|
|Predicted||Mouse, Chicken, Dog, C.Elegans, Horse, Pig, Monkey, Rabbit, Sheep|
|Other Names||T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E|
|Target/Specificity||A synthetic peptide aa156-168 (ERPPPVPNPDYEP) from human CD3e|
|Application Note||Flow Cytometry : 0.5-1ug/million cells|
Immunofluorescence : 1-2ug/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 1-2ug/500ug protein lysate.
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD3 epsilon Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses (PubMed:15546002, PubMed:8490660).|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Recognizes the -chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
1. Cibull ML et. al. Histopathology, 1989, 15(6):599-605.
2. Mason DY et. al. Journal of Clinical Pathology, 1989, 42(11):1194-200.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.