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Purified Mouse Monoclonal Antibody
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- BACKGROUND
Application 
| IHC, IF, ICC |
|---|---|
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Clone Names | NM97 |
| Target/Specificity | Nuclei of myeloid leukemia biopsy cells |
|---|---|
| Application Note | Immunofluorescence : 0.5-1.0 µg/ml Immunocytochemistry (Acetone-fixed cells) : 0.5-1.0 µg/ml for 30 minutes at RT Immunohistology (Frozen & Formalin-paraffin) : 0.5-1.0 µg/ml for 30 minutes at RT. |
| Format | 0.5 ml at 200ug/ml with BSA and azide |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Nuclear Membrane Marker Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM97 recognizes an antigen associated with the nuclear membrane expressed in human cells. It can be used to stain the nuclear membrane in cell or tissue preparations and can be used as a marker of the nuclear membrane in subcellular fractions. It produces a ring pattern around the nucleus of cells of normal and malignant cells and may be used to stain the nuclear membrane of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
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