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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >    NKX2.2 (NeuroenDOC - rine & Ewing's Sarcoma Marker) Antibody - With BSA and Azide   

NKX2.2 (NeuroenDOC - rine & Ewing's Sarcoma Marker) Antibody - With BSA and Azide

Purified Mouse Monoclonal Antibody

  •  -  NKX2.2 (NeuroenDOC - rine & Ewing's Sarcoma Marker) Antibody - With BSA and Azide AH10301
    Formalin-paraffin Ewing’s sarcoma stained with NKX2.2 Ab (NX2/294).
  •  -  NKX2.2 (NeuroenDOC - rine & Ewing's Sarcoma Marker) Antibody - With BSA and Azide AH10301
    Formalin-paraffin pancreas stained with NKX2.2 Ab (NX2/294).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95096
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b, kappa
Clone Names NX2/294
Calculated MW 40-50kDa
Additional Information
Gene ID 4821
Other Names Homeobox protein Nkx-22, Homeobox protein NK-2 homolog B, NKX2-2, NKX22, NKX2B
Target/Specificity Human NKX2.2 recombinant protein
Application Note ELISA : For coating, order Ab without BSA
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 1-2ug/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
Format 0.5 ml at 200ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions NKX2.2 (NeuroenDOC - rine & Ewing's Sarcoma Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NKX2-2
Synonyms NKX2.2, NKX2B
Function Acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108}
Research Areas
Citations (0)

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Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.


1. Wang, Y.C. et al. (2009) Endocr Relat Cancer 16, 267-79.
2. Yoshida A et. al. Am J Surg Pathol. 2012;36(7):993-9.

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$ 124.00
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$ 454.00
Cat# AH10301
(40 western blots)
Availability: 2-3 days
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