TGF-alpha (Transforming Growth Factor alpha) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC, FC, IP, E|
|Reactivity||Human, Zebrafish, Rabbit|
|Other Names||Protransforming growth factor alpha, Transforming growth factor alpha, TGF-alpha, EGF-like TGF, ETGF, TGF type 1, TGFA|
|Target/Specificity||A 10-amino acid synthetic peptide (aa34-43) from human TGFα.|
|Application Note||ELISA : For coating, order Ab without BSA|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Immunoprecipitation : 1-2ug/500ug protein
Western Blotting (Not tested)
Immunohistology (Frozen & Formalin-paraffin) : 1-2ug/ml for 30 min at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||TGF-alpha (Transforming Growth Factor alpha) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.|
|Cellular Location||Transforming growth factor alpha: Secreted, extracellular space|
|Tissue Location||Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines|
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Provided below are standard protocols that you may find useful for product applications.
This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGFα (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
1. Bebok Z; Szekeres G; Horvath G; Duda E; Nemeth P. [Creation of monoclonal antibodies against tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor alpha (TFG-alpha), their definition and possible use]. Orvosi Hetilap, 1993, 134(24):1303-7. Language: Hungarian.
2. Bebok Z; Markus B; Nemeth P. Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry. Breast Cancer Research and Treatment, 1994, 29(3):229-35.
3. Nasim, M. M., Thomas, D. M., Alison, M. R., and Fil]ipe, M. I. Transforming growth factor a expression in normal gastric mucosa, intestinal metaplasia, dysplasia and gastric carcinoma – an immunohistochemical study, Histopathology. 20: 339-343, 1992.
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