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PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody - With BSA and Azide

Purified Mouse Monoclonal Antibody

  •  -  PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody - With BSA and Azide AH10312
    Formalin-fixed, paraffin-embedded human human tonsil stained with PCNA MAb (PC10)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12004
Other Accession P17918, P04961
Reactivity Human, Mouse, Rat
Predicted Zebrafish, Chicken, Pig, Monkey, Yeast
Host Mouse
Clonality Monoclonal
Isotype IgG2a, kappa
Clone Names PC10
Calculated MW 36kDa
Additional Information
Gene ID 5111
Other Names Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Target/Specificity Rat PCNA/Protein A fusion protein
Application Note ELISA : For coating, order Ab without BSA
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1.0 µg/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 0.5-1 µg/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 0.5-1.0 µg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
Format 0.5 ml at 200ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Cellular Location Nucleus. Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
Research Areas
Citations (0)

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Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.


1. Waseem NH & Lane DP. 1990. J Cell Sci. 96:121-9.
2. Hall PA et al. 1990. J. Pathol. 162(4):285-94.
3. Landberg G & Roos G. 1991. Cancer Res. 51 (17):4570-4.
4. Woods AL et al. 1991. Histopathol. 19(1):21-7
5. Yu,CC. et al. 1991. Histopathol. 19(1):29-33.

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$ 124.00
$ 324.00
$ 454.00
Cat# AH10312
(40 western blots)
Availability: 2-3 days
Bulk Size
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