Progesterone Receptor (Marker of Progestin Dependence) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC|
|Calculated MW||PR-A (81kDa) and PR-B (116kDa).|
|Other Names||Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3, PGR, NR3C3|
|Target/Specificity||Recombinant human Progesterone Receptor protein|
|Application Note||Western Blotting (0.5-1.0 µg/ml for 2 hours at Room Temp)|
Immunohistology (Frozen & formalin-fixed) : 0.5-1.0 µg/ml for 30 min at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Progesterone Receptor (Marker of Progestin Dependence) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.|
|Cellular Location||Nucleus. Cytoplasm. Note=Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases Isoform 4: Mitochondrion outer membrane|
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Provided below are standard protocols that you may find useful for product applications.
This MAb is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone. It is excellent for immunohistochemical staining of formalin/paraffin tissues.
1. Press M, et al. Steroids. 2002 Aug; 67(9):799-813.
2. Mote P, et al. J Clin Pathol., 2001; 54: 624-630.
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