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Phosphotyrosine (P-Tyr) Antibody - With BSA and Azide

Purified Mouse Monoclonal Antibody

     
  •  -  Phosphotyrosine (P-Tyr) Antibody - With BSA and Azide AH10323
    Formalin-fixed, paraffin-embedded breast carcinoma stained with Phosphotyrosine Ab (PY20). Note cell surface and cytoplasmic staining.
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, IP, E
Reactivity Human, Mouse, Rat
Predicted Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names PY20
Calculated MW Depends upon the phosphorylated target KDa
Additional Information
Target/Specificity Phosphotyrosine conjugated to KLH
Application Note ELISA : For coating, order Ab without BSA
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 1-2ug/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 1-2ug/ml for 30 minutes at RT
(No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues).
Format 0.5 ml at 200ug/ml with BSA and azide
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
Precautions Phosphotyrosine (P-Tyr) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)

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Background

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

References

1. Glenny, Jr. JR, et. al. Journal of Immunological Methods, 1988, 109:277-85.

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$ 124.00
$ 324.00
$ 454.00
Cat# AH10323
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