|Application ||WB, IHC, FC, IP, E|
|Reactivity||Human, Mouse, Rat|
|Predicted||Human, Mouse, Rat|
|Calculated MW||Depends upon the phosphorylated target KDa|
|Target/Specificity||Phosphotyrosine conjugated to KLH|
|Application Note||ELISA : For coating, order Ab without BSA|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western Blotting : 0.5-1.0 µg/ml
Immunoprecipitation : 1-2ug/500ug protein lysate
Immunohistology (Frozen & Formalin-fixed) : 1-2ug/ml for 30 minutes at RT
(No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Phosphotyrosine (P-Tyr) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
1. Glenny, Jr. JR, et. al. Journal of Immunological Methods, 1988, 109:277-85.
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