|Application ||WB, IHC, IF, FC, IP, E|
|Other Names||T-cell surface glycoprotein CD1b, CD1b, CD1B|
|Target/Specificity||Human peripheral lymphocytes|
|Application Note||ELISA : For coating, order Ab without BSA|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1.0 µg/ml
Functional Studies : Order Ab without BSA & Azide
Immunoprecipitation : 0.5-1 µg/500ug protein lysate
Immunohistology (Frozen) (0.5-1.0 µg/ml for 30 minutes at RT).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD1b (T-Cell Surface Glycoprotein) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.|
|Cellular Location||Cell membrane; Single- pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single- pass type I membrane protein. Note=Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes.|
|Tissue Location||Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues|
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Provided below are standard protocols that you may find useful for product applications.
The mouse monoclonal antibody recognizes CD1b, a 44kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells. The CD1 multigene family encodes five forms of the CD1 T-cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. Constitutive endocytosis of CD1B molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. CD1B is also expressed in interdigitating cells.
1. Knapp W. et al. (eds) Leukocyte Typing IV, p251-263, Oxford University Press, Oxford, 1989
2. Maher JK and Kronenberg M. The role of CD1 molecules in immune responses to infection. Curr Opin Immunol 1997, 9(4):456-461
3. Blumberg RS et al. Structure and function of the CD1 family of MHC-like cell surface proteins. Immunol Rev 1995, 147:5-29.
4. Salamone MC et al. Analysis of CD1 molecules on haematological malignancies of myeloid and lymphoid origin. II. Intracellular detection of CD1 antigens. Dis Markers 1990, 8(5):275-281.
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