|Application ||IHC, IF, FC, ICC|
|Target/Specificity||Hepatocytes from 20-22 week-old human fetus|
|Application Note||Flow Cytometry : 0.5-1ug/million cells|
Immunofluorescence : 0.5-1.0 µg/ml
Immunocytochemistry (Frozen) (0.5-1.0 µg/ml for 30 minutes at RT)
Immunohistology (Frozen) (0.5-1.0 µg/ml for 30 minutes at RT).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Erythrocyte Specific Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Mouse Anti-Human Erythrocytes Clone SFL23.6 MAb is directed against an erythroid cell surface antigen. It shows a well defined reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. Although the exact identity of the antigen has yet to be determined, it has been shown to be distinct from Glycophorin A. This MAb is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.
1. Das Gupta, A., Samoszuk, MKI, Papayannopoulou, T, and Stamatoyannopoulos, G: SFL 23.6: A monoclonal antibody reactive with CFU-E, erythroblasts, and erythrocytes. Blood 66:522-526, 1985.
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