Involucrin (Squamous Cell Terminal Differentiation Marker) Antibody - With BSA and Azide
Purified Mouse Monoclonal Antibody
|Application ||WB, IHC, FC, IP, E|
|Other Accession||P18174, P18175|
|Other Names||Involucrin, IVL|
|Target/Specificity||Purified involucrin from human keratinocytes|
|Application Note||ELISA : For coating, order antibody without BSA|
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Immunoprecipitation : 1-2ug/500ug protein
Western Blotting : 0.5-1.0 µg/ml
Immunohistology (Frozen & formalin-fixed) : 1-2ug/ml for 30 min at RT
(Staining of formalin-fixed tissues is ENHANCED by digestion with trypsin (or Protease XXV from Sigma) at 1mg/ml PBS for 5 min at 37°C]).
|Format||0.5 ml at 200ug/ml with BSA and azide|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||Involucrin (Squamous Cell Terminal Differentiation Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.|
|Cellular Location||Cytoplasm. Note=Constituent of the scaffolding of the cornified envelope|
|Tissue Location||Keratinocytes of epidermis and other stratified squamous epithelia|
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Provided below are standard protocols that you may find useful for product applications.
It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
1. Hudson DL, et. al. Hybridoma, 1992, 11:367-79.
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